Journal article

Sulfate activation and transport in mammals: system components and mechanisms

NB Schwartz, S Lyle, JD Ozeran, H Li, A Deyrup, K Ng, J Westley

Chemico-Biological Interactions | ELSEVIER SCI IRELAND LTD | Published : 1998


Extensive studies on the mammalian sulfate-activating enzymes and PAPS translocase have enhanced our understanding of the overall pathway of sulfate activation and utilization. Isolation of the PAPS-synthesizing activities from rat chondrosarcoma and preparation of stable non-hydrolyzable analogs of APS and PAPS have facilitated the kinetic characterization of mammalian ATP sulfurylase and APS kinase. These studies provided the basis for further experimental work showing that APS, the labile intermediate product, is channeled directly between the sulfurylase and kinase active sites. The defect in the brachymorphic mutant mouse lies in this channeling mechanism, thus interfering with efficien..

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University of Melbourne Researchers