Journal article

THE MAJOR RADIOIODINATED CUTICULAR ANTIGENS OF ONCHOCERCA-GIBSONI MICROFILARIAE ARE NEITHER SPECIES NOR ONCHOCERCA SPECIFIC

KP FORSYTH, DB COPEMAN, RF ANDERS, GF MITCHELL

ACTA TROPICA | ELSEVIER SCIENCE BV | Published : 1981

Abstract

The possible role of microfilarial surface (cuticular) antigens in immuno-diagnosis of human filarial infections has been assessed using microfilariae (mf) of the cattle parasite Onchocerca gibsoni. A Triton X-100 extract of 125I-labeled O. gibsoni mf was reacted with a panel of sera from humans infected with Onchocerca volvulus, Wuchereria bancrofti and Schistosoma japonicum as well as sera from uninfected controls. Results of these immunoprecipitations indicated that sera from humans infected with O. volvulus or W bancrofti contain antibody specificities recognising certain of the radioiodinated cuticular proteins of O. gibsoni mf. Two-dimensional gel analysis and subsequent autoradiograph..

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University of Melbourne Researchers