Journal article

Post-Translational Modifications and Protein-Specific Isoforms in Endometriosis Revealed by 2D DIGE

Andrew N Stephens, Natalie J Hannan, Adam Rainczuk, Katie L Meehan, Jenny Chen, Peter K Nicholls, Luk JF Rombauts, Peter G Stanton, David M Robertson, Lois A Salamonsen

Journal of Proteome Research | AMER CHEMICAL SOC | Published : 2010

Abstract

Endometriosis is a chronic disorder affecting approximately 10% of women in whom endometrial tissue forms painful lesions outside the uterus. It has a major impact on their physical, mental and social well-being but has no known cure, and there is no nonsurgical means of diagnosis. We have used a proteomic approach to identify proteins with altered abundance in the eutopic endometrium of endometriosis patients in the midsecretory phase of the menstrual cycle. 2D-differential in gel electrophoresis (DIGE) and mass spectrometry identified 20 proteins that were present at different levels in endometriosis patients (p < 0.05), many of which have not previously been associated with endometriosis...

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