Post-Translational Modifications and Protein-Specific Isoforms in Endometriosis Revealed by 2D DIGE
Andrew N Stephens, Natalie J Hannan, Adam Rainczuk, Katie L Meehan, Jenny Chen, Peter K Nicholls, Luk JF Rombauts, Peter G Stanton, David M Robertson, Lois A Salamonsen
Journal of Proteome Research | AMER CHEMICAL SOC | Published : 2010
Endometriosis is a chronic disorder affecting approximately 10% of women in whom endometrial tissue forms painful lesions outside the uterus. It has a major impact on their physical, mental and social well-being but has no known cure, and there is no nonsurgical means of diagnosis. We have used a proteomic approach to identify proteins with altered abundance in the eutopic endometrium of endometriosis patients in the midsecretory phase of the menstrual cycle. 2D-differential in gel electrophoresis (DIGE) and mass spectrometry identified 20 proteins that were present at different levels in endometriosis patients (p < 0.05), many of which have not previously been associated with endometriosis...View full abstract
Awarded by National Health and Medical Research Council of Australia
We thank Cassie Hincks, Jin Zhan, and Ming Lee for technical assistance and Dianne Arnold for assistance in preparation of the manuscript. Funding for this study was provided by Schering AG (Berlin), the Ovarian Cancer Research Foundation and by the National Health and Medical Research Council of Australia (Program Grant 494802, Fellowships (L.A.S. 388901, D.M.R. 494803).