Journal article
Depletion of high-abundance serum proteins from human uterine lavages enhances detection of lower-abundance proteins
NJ Hannan, CJ Stoikos, AN Stephens, LA Salamonsen
Journal of Proteome Research | AMER CHEMICAL SOC | Published : 2009
DOI: 10.1021/pr800811y
Abstract
Two-dimentional electrophoresis, 2-DE, of human uterine lavages demonstrated the abundance of serum proteins. Two methods of serum protein removal were assessed. Multiple affinity removal system was most effective in depleting abundant serum proteins. 2D-DiGE comparing depleted versus nondepleted lavages resulted in increased protein detection following depletion, further enhanced by assessing the protein profile over a narrower pl 4-7 range. Demonstrating depletion of abundant serum proteins is important for sensitive proteomic analysis. © 2009 American Chemical Society.
Grants
Awarded by National Health and Medical Research Council of Australia
Funding Acknowledgements
The authors acknowledge Associate Professor David Robertson and Dr. Peter Stanton for technical advice, Sister Judi Hocking, Doctors Mark Lawrence, Beverley Vollenhoven, Luk Rombauts, for collecting the lavages samples, and the patients who provided their consent. This study was supported by the National Health and Medical Research Council of Australia (grant no. 241000; no. 143798 and Postgraduate Biomedical Research Scholarship) and Schering Research Grant (RANZCOG).