Host-protective fragments and antibody binding epitopes of the Taenia ovis 45W recombinant antigen

Abstract

The protective efficacy of a recombinant Taenia ovis vaccine antigen, 45W, was compared in sheep vaccine trials with antigen expressed by the full length 45W cDNA and by incomplete copies of the cDNA. Vaccine trials were also carried out using antigen expressed by a cDNA (45S) having a sequence similar, but not identical, to 45W. Stability of the 45W antigen expressed in Escherichia coli was found to be increased after deletion of cDNA sequence encoding 19 COOH-terminal amino acids. This truncated form of the antigen was designated 45W B/X. Vaccination of sheep with antigen expressed by 45W, 45W B/X, as well as full length 45W and 45S cDNAs, induced high levels of protection. Vaccination wit..