Journal article

Immunodiagnosis of alveolar echinococcosis by enzyme-linked immunosorbent assay using a partially purified Em18/16 enriched fraction

A Ito, L Ma, M Itoh, SY Cho, Y Kong, SY Kang, T Horii, XL Pang, M Okamoto, T Yamashita, MW Lightowlers, XG Wang, YH Liu

CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY | AMER SOC MICROBIOLOGY | Published : 1997

Abstract

An improved enzyme-linked immunosorbent assay (ELISA) system using partially purified Eml8/16 enriched fraction (PP-Em18/16) prepared by isoelectric focusing was evaluated for serodiagnosis of alveolar echinococcosis (AE). The PP-Em18/16-ELISA was compared with Em2plus-ELISA by using sera from AE and cystic echinococcosis (CE) patients in China, where both AE and CE are endemic; sera from CE patients in Australia, where only CE exists; and sera from patients with cysticercosis, paragonimiasis, or sparganosis in Korea, where no indigenous AE or CE exists. We used Em2plus-ELISA as a standard ELISA and found 24.6% (17 of 69 specimens) cross-reactivity with sera from CE. Furthermore, some of the..

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