Journal article

Sequence analysis of a gene family encoding Taenia ovis vaccine antigens expressed during embryogenesis of eggs

J Waterkeyn, C Gauci, A Cowman, M Lightowlers

Molecular and Biochemical Parasitology | ELSEVIER SCIENCE BV | Published : 1997


The 45W vaccine antigen of the cestode parasite Taenia ovis is a member of a small gene family estimated to comprise six members. All six genes were cloned and characterised. Nucleotide sequence analysis of the different family members identified a set of closely related genes exhibiting between 75.2 and 98.6% DNA sequence homology. Intron/exon structure for the various genes constituting the family was found to be conserved. Open reading frame analysis and pairwise alignments of predicted polypeptides from the various members of the family revealed that the encoded proteins share between 51.8 and 96.5% amino acid identity with the host-protective 45W antigen. Polymerase chain reaction (PCR)..

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