Vaccination against Taenia taeniaeformis infection in rats using a recombinant protein and preliminary analysis of the induced antibody response

Abstract

Primary screening of a cDNA expression library of Taenia taeniaeformis oncospheres in λgt11 bacteriophage was carried out using rabbit anti-T. taeniaeformis oncosphere serum affinity-purified from disrupted oncosphere pellets. From approximately 1.6 × 105 plaques, 21 single clones that were positive with the affinity-purified antibodies were isolated. Sibling analysis revealed that 17 clones out of the 21 could be assigned to five different antigen families. Only family 1 was strongly recognized by a serum prepared in a rabbit against a partially purified host-protective oncosphere antigen fraction. The fragments of λDNA were inserted into a pGEX plasmid vector that encodes glutathione S-tra..