Journal article
Antigen-specific IgA-secreting cells induced by peripheral vaccination
JS Rothel, LA Corner, HF Seow, PR Wood, MW Lightowlers
Immunology and Cell Biology | BLACKWELL SCIENCE | Published : 1996
DOI: 10.1038/icb.1996.52
Abstract
Antigen-specific IgA-secreting cells were shown to be generated in peripheral lymph nodes following s.c. vaccination. The efferent duct of prefemoral lymph nodes of sheep were cannulated prior to vaccination at a site draining to the cannulated node. Vaccines were contained a recombinant protein (Taenia ovis 45W-GST) and either incomplete Freund's, Quil A or Al(OH)3 as adjuvant. Lymph fluid was examined for the presence of 45W-GST- specific antibody by ELISA and antibody-secreting cells by enzyme-linked immunospot (ELISPOT) assay. Large numbers of anti-45W-GST IEA-secreting cells were detected at various times after vaccination as were IgM, IgG1 and IgG2-secreting cells. For sheep vaccinated..
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