Journal article

CHARACTERIZATION OF A MAJOR HEMAGGLUTININ PROTEIN FROM MYCOPLASMA-GALLISEPTICUM

PF MARKHAM, MD GLEW, MR BRANDON, ID WALKER, KG WHITHEAR

Infection and Immunity | AMER SOC MICROBIOLOGY | Published : 1992

Abstract

Mycoplasma gallisepticum cell membranes were used to immunize mice to produce monoclonal antibodies to cell surface proteins. Three monoclonal antibodies were chosen for further characterization. All three reacted in immunoblots with an M. gallisepticum protein band of M(r) approximately 67,000 (designated pMGA). By using immunoelectron microscopy, pMGA was shown to be located on the cell surface. When M. gallisepticum whole cells were treated with up to 250 micrograms of trypsin per ml for 30 min, the only major protein lost from the cell surface as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by Western immunoblot transfer was pMGA. Two of the pMGA-specific ..

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