Journal article

Characterisation of monoclonal antibodies to ovine interleukin-6 and the development of a sensitive capture ELISA

P McWaters, L Hurst, PJ Chaplin, RA Collins, PR Wood, JPY Scheerlinck

Veterinary Immunology and Immunopathology | ELSEVIER SCIENCE BV | Published : 2000


A purified recombinant ovine (rOv) interleukin-6 (IL-6) was used to generate specific murine monoclonal antibodies (mAbs) and a polyclonal rabbit antisera to this cytokine. From the 31 initial hybridoma cell lines generated, three stable clones were established which secreted mAbs to rOvIL-6, as judged by a direct enzyme-linked immunosorbent assay (ELISA) and Western blotting. Their specificity was further confirmed by demonstrating that none of the mAbs recognised any of the six other irrelevant recombinant ovine cytokines tested by direct ELISA. All three mAbs displayed cross-reactivity with human and African green monkey IL-6 as demonstrated by direct ELISA and Western blotting. In contra..

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