Tuberculin anergy in clinically normal individuals: I. lymphokine and lymphocyte transformation studies

Abstract

Tuberculin anergy was demonstrated in a number of clinically normal individuals who, on being immunized with Bacillus Calmette-Guerin (BCG), failed to demonstrate a positive Mantoux skin test. Lymphoid cells from these anergic subjects when stimulated with purified protein derivative (PPD) in vitro did not produce macrophage migration inhibition factor (MIF). Lymphocyte transformation studies demonstrated that while Mantoux-posttive, non-BCG immunized Mantoux-negative and anergic individuals could all undergo blast cell transformation in the presence of PH A, only lymphoid cells from Manloux-positive and a proportion of anergic people were capable of blast cell transformation when cultured w..