Differential activation of platelets from normal and allergic ponies by PAF and ADP

Abstract

Objective and Design: Altered platelet responsiveness has been demonstrated in human atopic dermatitis. This study has compared the in vitro function of platelets from normal ponies and those with the allergic skin disease, sweet itch. Subjects: Ponies with a clinical history of sweet itch and normal ponies were used as blood donors. Methods: PAF and ADP-induced platelet aggregation was measured and TxB2 production quantitated at the time of maximal aggregation; 12-HETE was additionally measured in some samples. Agonist-induced release of 3[H]5-HT was also studied. Results: Although both PAF and ADP caused equine platelet aggregation, only PAF stimulated eicosanoid and 5-HT release. There we..