Current status of IVM/IVF and embryo culture in humans and farm animals

Abstract

Human oocytes are usually recovered from preovulatory follicles of women treated with gonadotropins and gonadotropin releasing hormone analogue. The oocytes are usually mature and are inseminated within 4 to 6 hours of aspiration from follicles. High rates of fertilization occur without any particular treatment of washed sperm. Failure of fertilization due to poor quality sperm is very effectively treated by microinjection of sperm directly into the cytoplasm of the mature oocyte. Fertilized oocytes may be cultured in a wide range of media and the cleaving embryos transferred within 48 to 72 hours to the uterus. Coculture methods have been reported for culture of human embryos including huma..