Journal article

AMINO-ACIDS AND AMMONIUM REGULATE MOUSE EMBRYO DEVELOPMENT IN CULTURE

DK GARDNER, M LANE

BIOLOGY OF REPRODUCTION | SOC STUDY REPRODUCTION | Published : 1993

Abstract

The regulation of 1-cell mouse embryo development in culture by amino acids was investigated. When the 20 amino acids in Eagle's medium were present, blastocyst formation at 72 h (9%; p < 0.01), and blastocyst cell number (66; p < 0.05) and hatching (45%; p < 0.05) after 96 h of culture were significantly increased, compared to control embryos grown in the absence of amino acids (0%, 60, and 23%, respectively). The beneficial effect of Eagle's amino acids was attributed primarily to the non-essential group. In the presence of non-essential amino acids, blastocyst formation (54%; p < 0.001) and cell number after 72 h of culture (33; p < 0.05), and blastocyst cell number (69; p < 0.01) and hat..

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University of Melbourne Researchers