Journal article

STRUCTURAL-ANALYSIS OF THE CARBOHYDRATE MOIETY OF ARABINOGALACTAN-PROTEINS FROM STIGMAS AND STYLES OF NICOTIANA-ALATA

AM GANE, D CRAIK, SLA MUNRO, GJ HOWLETT, AE CLARKE, A BACIC

Carbohydrate Research | ELSEVIER SCIENCE BV | Published : 1995

Abstract

Arabinogalactan-proteins (AGPs) from the female reproductive tissues (stigmas and styles) of Nicotiana alata were isolated from the saturated ammonium sulfate supernatant of buffer-soluble extracts by precipitation with the beta-glucosyl Yariv reagent, followed by gel-filtration chromatography under dissociating conditions. The AGPs had characteristics typical of other AGPs: a high proportion of carbohydrate (95%) with a high ratio of Gal p to Ara f (2:1), and a low protein content (5%) with high levels of alanine, serine, and hydroxyproline. The AGPs consisted of a major species which was almost neutral, and a minor species which was more negatively charged. Sedimentation equilibrium experi..

View full abstract

University of Melbourne Researchers