Glucosinolates in the pollen of rapeseed and indian mustard

Abstract

The glucosinolate composition of the pollen from rapeseed (Brassica napus cv Midas) and Indian mustard (B.juncea. cv Early Yellow) was investigated by HPLC. Rapeseed pollen was found to contain significant amounts of 2-hydroxybut-3-enyl-, 2-hydroxypent-4-enyl-, pent-4-enyl-, and 2-phenylethylglucosinolates. Trace amounts of but-3-enyl-, 3-indolylmethyl-, 4-methoxy-3-indolylmethyl-, and 1-methoxy-3-indolylmethylglucosinolates were also observed. Indian mustard pollen contained predominantly prop-2-enylglucosinolate, with minor amounts of 2-hydroxybut-3-enyl-, 2-hydroxypent-4-enyl-, but-3-enyl- and 2-phenylethylglucosinolates. Structural confirmation by GC/MS was obtained for three major rapes..