Complementation of areA- regulatory gene mutations of Aspergillus nidulans by the heterologous regulatory gene nit-2 of Neurospora crassa.
MA Davis, MJ Hynes
Proceedings of the National Academy of Sciences of the United States of America | Published : 1987
Loss-of-function mutations in the regulatory gene areA of Aspergillus nidulans prevent the utilization of a wide variety of nitrogen sources. The phenotypes of nit-2 mutants of Neurospora crassa suggest that this gene may be analogous to the areA gene. Transformation has been used to introduce a plasmid containing the nit-2 gene into A. nidulans. The nit-2 gene of Neurospora complemented mutations in the areA gene, restoring the ability to use a variety of nitrogen sources. This indicated that the activator function of nit-2 and areA gene products was retained across these two fungal species. Southern blot analysis revealed both single-copy and multicopy integrations and, in at least one cas..View full abstract