Pre-differentiation of human neural stem cells into GABAergic neurons prior to transplant results in greater repopulation of the damaged brain and accelerates functional recovery after transient ischemic stroke
Hima CS Abeysinghe, Laita Bokhari, Anita Quigley, Mahesh Choolani, Jerry Chan, Gregory J Dusting, Jeremy M Crook, Nao R Kobayashi, Carli L Roulston
STEM CELL RESEARCH & THERAPY | BMC | Published : 2015
INTRODUCTION: Despite attempts to prevent brain injury during the hyperacute phase of stroke, most sufferers end up with significant neuronal loss and functional deficits. The use of cell-based therapies to recover the injured brain offers new hope. In the current study, we employed human neural stem cells (hNSCs) isolated from subventricular zone (SVZ), and directed their differentiation into GABAergic neurons followed by transplantation to ischemic brain. METHODS: Pre-differentiated GABAergic neurons, undifferentiated SVZ-hNSCs or media alone were stereotaxically transplanted into the rat brain (n=7/group) 7 days after endothelin-1 induced stroke. Neurological outcome was assessed by neuro..View full abstract
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Awarded by National Health and Medical Research Council Australia (NHMRC)
Awarded by NHMRC
The authors thank Dr Yiping Fan for assisting in the procurement of primary human brain tissue utilized for this study. The authors would also like to thank Professor Robin McAllen for producing the glass capillaries that were used for conducting stem cell transplant procedures. In addition, the authors would like to acknowledge The O'Brien Institute for the use of their facilities to conduct experimental procedures within the first two years of commencing this study. This research was supported by the National Health and Medical Research Council Australia (NHMRC; project grant #628767) and a NHMRC Principal Research Fellowship to GJD (#1003113). All statistical analyses were conducted in consultation with statistical consultant Rachel Sore of the Statistical Consulting Centre University of Melbourne, Victoria, Australia. The authors would like to dedicate this paper to the late Dr Nao Kobayashi, a remarkable colleague and cherished friend. Nao's passion and dedication to her scholarship was unparalleled and her friendship and caring spirit will be missed.