Intracellular Distribution of Fluorescent Copper and Zinc Bis(thiosemicarbazonato) Complexes Measured with Fluorescence Lifetime Spectroscopy
James L Hickey, Janine L James, Clare A Henderson, Katherine A Price, Alexandra I Mot, Gojko Buncic, Peter J Crouch, Jonathan M White, Anthony R White, Trevor A Smith, Paul S Donnelly
Inorganic Chemistry | AMER CHEMICAL SOC | Published : 2015
The intracellular distribution of fluorescently labeled copper and zinc bis(thiosemicarbazonato) complexes was investigated in M17 neuroblastoma cells and primary cortical neurons with a view to providing insights into the neuroprotective activity of a copper bis(thiosemicarbazonato) complex known as Cu(II)(atsm). Time-resolved fluorescence measurements allowed the identification of the Cu(II) and Zn(II) complexes as well as the free ligand inside the cells by virtue of the distinct fluorescence lifetime of each species. Confocal fluorescent microscopy of cells treated with the fluorescent copper(II)bis(thiosemicarbazonato) complex revealed significant fluorescence associated with cytoplasmi..View full abstract
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Australian Research Council, National and Health and Medical Research Council (Australia). P.S.D. is an ARC Future Fellow.