Transmission of a Bacillus thuringiensis cry3Aa transgene from diploid to tetraploid potato using 4x-2x hybridization: Effect of ploidy increase on transgene expression and implications for TPS hybrid production

Abstract

The study examined the effect of ploidy elevation through unreduced gametes on transgene expression in potato. Tetraploid transgenic progenies were obtained from one tetraploid potato cultivar crossed with 2n pollen producing diploid clones harbouring an exogenous transgene (cry3Aa). Both single- and multiple-insert diploid transgenic lines that were regenerated by Agrobacterium tumefaciens leaf disc inoculation were used in crosses. A DAS-ELISA system and no-choice feeding bioassay enabled characterization of the parental lines as either 'high' or 'low' expressers of the Cry3Aa protein. High Cry3Aa expression was observed for both single-insert transgenic diploids and their 4x-2x progeny. O..