Journal article

LOSS OF A HISTIDINE RESIDUE AT THE ACTIVE-SITE OF S-LOCUS RIBONUCLEASE IS ASSOCIATED WITH SELF-COMPATIBILITY IN LYCOPERSICON-PERUVIANUM

J ROYO, C KUNZ, Y KOWYAMA, M ANDERSON, AE CLARKE, E NEWBIGIN

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | NATL ACAD SCIENCES | Published : 1994

Abstract

Gametophytic self-incompatibility in the Solanaceae is controlled by a single, multiallelic locus, the S locus. We have recently described an allele of the S locus of Lycopersicon peruvianum that caused this normally self-incompatible plant to become self-compatible. We have now characterized two glycoproteins present in the styles of self-compatible and self-incompatible accessions of L. peruvianum: one is a ribonuclease that cosegregates with a functional self-incompatibility allele (S6 allele); the other cosegregates with the self-compatible allele (Sc allele) but has no ribonuclease activity. The derived amino acid sequences of the cDNAs encoding the S6 and Sc glycoproteins resemble sequ..

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