Pea convicilin: structure and primary sequence of the protein and expression of a gene in the seeds of transgenic tobacco

Abstract

Convicilin, a trimeric globulin of pea (Pisum sativum L.) seeds, is closely related to vicilin and composed of polypeptides of 68.2 kilodaltons. A partial copy DNA (cDNA) clone encoding convicilin was isolated, sequenced, and used to select a convicilin gene from a pea genomic library. A part of the genomic clone was sequenced to obtain the coding sequences missing in the cDNA clone and a further 1 kilobase 5′ to the start of transcription were also obtained. The entire sequence of convicilin was deduced from the combined genomic and cDNA sequences. The complete gene encoding convicilin was transferred to tobacco (Nicotiana tabacum L.) and the characteristics of its expression in the seeds o..