Journal article

Phylogenetic analyses and expression studies reveal two distinct groups of calreticulin isoforms in higher plants

S Persson, M Rosenquist, K Svensson, R Galvao, WF Boss, M Sommarin

Plant Physiology | AMER SOC PLANT BIOLOGISTS | Published : 2003

Abstract

Calreticulin (CRT) is a multifunctional protein mainly localized to the endoplasmic reticulum in eukaryotic cells. Here, we present the first analysis, to our knowledge, of evolutionary diversity and expression profiling among different plant CRT isoforms. Phylogenetic studies and expression analysis show that higher plants contain two distinct groups of CRTs: a CRT1/CRT2 group and a CRT3 group. To corroborate the existence of these isoform groups, we cloned a putative CRT3 ortholog from Brassica rapa. The CRT3 gene appears to be most closely related to the ancestral CRT gene in higher plants. Distinct tissue-dependent expression patterns and stress-related regulation were observed for the i..

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University of Melbourne Researchers