Conference Proceedings

SELF-INCOMPATIBILITY - INSIGHTS THROUGH MICROSCOPY

GI MCFADDEN, MA ANDERSON, I BONIG, JE GRAY, AE CLARKE

JOURNAL OF MICROSCOPY | WILEY-BLACKWELL | Published : 1992

Abstract

The contributions of microscopy to our understanding of gametophytic self‐incompatibility in the ornamental tobacco Nicotiana alata are reviewed. Self‐incompatibility is a genetically based system which prevents self‐fertilization and thereby promotes outbreeding in plants. Pollen bearing an S‐allele identical to either of the S‐alleles in the female sporophytic tissues is rejected. Several S‐allele‐specific style glycoproteins have been isolated from Solanaceous species. All have ribonuclease activity and are termed S‐RNases. The cDNA clones encoding N. alata S‐RNases have been used in in situ hybridization experiments which show that the genes are expressed in the transmitting tract of the..

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