Journal article
A simple fixation and embedding method for use in hybridization histochemistry on plant tissues
GI McFadden, I Bonig, EC Cornish, AE Clarke
Histochemical Journal | SPRINGER | Published : 1988
DOI: 10.1007/BF01002612
Abstract
A simple method for fixing and embedding plant tissue for hybridization histochemistry has been developed. Material is fixed with paraformaldehyde-glutaraldehyde by the phase-partition technique and embedded in LR Gold. The method preserves nucleic acids in situ. Double-stranded32P-labelled DNA probes hybridized to complementary RNAs in thin and semithin sections. Biotinylated probes detected with immunogold markers have been used to localize transcripts at the electron nucroscopic level. © 1988 Chapman and Hall Ltd.