A multilocus, temperature stress-related gene expression profile assay in Acropora millepora, a dominant reef-building coral
P Souter, LK Bay, N Andreakis, N Csaszar, FO Seneca, MJH van Oppen
Molecular Ecology Resources | WILEY | Published : 2011
We report an accurate multiplex reverse transcription quantitative polymerase chain reaction (RT-qPCR) assay, capable of reproducing gene expression profiles from 16 target genes [12 genes of interest (GOIs) and four reference genes (RGs)] in Acropora millepora, a common reef-building model coral species. The 12 GOIs have known or putative roles in the coral bleaching response, yet the method is not restricted to this particular assay and gene set. The procedure is based on the Beckman Coulter (Fullerton, CA, USA) GenomeLab™ GeXP Genetic Analysis System and bridges the gap between quantitative real-time PCR (qPCR) expression analysis of a single or a small number of genes and microarray gene..View full abstract
This study was conducted using samples collected under G18792.1 GBRMPA permit. We thank M Matz and E Meyer for access to their transcriptomic data before it was publicly available (http://www.bio.utexas.edu/research/matz_lab/matzlab/Data.html). This project was funded by the Australian Government's Marine and Tropical Sciences Research Facility, the Australian Institute of Marine Science and the ARC Centre of Excellence for Coral Reef Studies.