Transcriptomic variation in a coral reveals pathways of clonal organisation
Line K Bay, H Bjorn Nielsen, Hanne Jarmer, Francois Seneca, Madeleine JH van Oppen
Marine Genomics | ELSEVIER | Published : 2009
A microarray study was undertaken to examine the potential for clonal gene expression variation in a branching reef building coral, Acropora millepora. The role of small-scale gradients in light and water flow was examined by comparing gene expression levels between branch elevation (tip and base) and position (centre and edge) of replicate coral colonies (n=3). Analyses of variance revealed that almost 60% of variation in gene expression was present between colonies and 34 genes were considered differentially expressed between colonies (minimum P=6.5×10(-4)). These genes are associated with energy metabolism, protein biosynthesis and cell-cell recognition representing either genotypic varia..View full abstract
We are thankful for the use of the 18K A. millepora microarray developed by the ARC Centre for the Molecular Genetics of Development at ANU. Raechel Littman helped with fieldwork and RNA extractions and Andrew Baird provided useful comments on the manuscript. This research was conducted at the Australian Institute of Marine Science with funding provided by the ARC Centre of Excellence for Coral Reef Studies, the Queensland Government and the Marine and Tropical Science Research Facility.