Journal article

Regulation of stromal sedoheptulose-1,7-bisphosphatase activity and its role in controlling the reductive pentose phosphate pathway of photosynthesis

IE Woodrow, DA Walker

BBA - Bioenergetics | Published : 1983


The activation and steady-state kinetics of a wheat sedoheptulose-bisphosphatase, shown to be located in the chloroplast, are examined. Enzyme activation in the presence of the reductant, dithiothreitol, is slow and modulated by the sedoheptulose 1,7-bisphosphate concentration. A mechanism for the regulation of enzyme activity is proposed. In this scheme, both the enzyme-substrate complex and the enzyme alone undergo a slow reductive activation and oxidative inactivation. The proportion of enzyme in the active form is governed by the reductant / oxidant ratio and the sedoheptulose 1,7-bisphosphate concentration. Control of sedoheptulose-bisphosphatase activity by these two factors is discuss..

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University of Melbourne Researchers

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