Chemokine Signaling Controls Integrity of Radial Glial Scaffold in Developing Spinal Cord and Consequential Proper Position of Boundary Cap Cells
Yan Zhu, Tomoko Matsumoto, Takashi Nagasawa, Fabienne Mackay, Fujio Murakami
Journal of Neuroscience | SOC NEUROSCIENCE | Published : 2015
Radial glial cells are the neural progenitors of the developing CNS and have long radial processes that guide radially migrating neurons. The integrity of the radial glial scaffold, in particular proper adhesion between the endfeet of radial processes and the pial basement membrane (BM), is important for the cellular organization of the CNS, as indicated by evidence emerging from the developing cortex. However, the mechanisms underlying the maintenance of radial glial scaffold integrity during development, when the neuroepithelium rapidly expands, are still poorly understood. Here, we addressed this issue in the developing mouse spinal cord. We show that CXCR4, a receptor of chemokine CXCL12..View full abstract
Awarded by Ministry of Education, Culture, Science and Technology, Japan
Awarded by Grants-in-Aid for Scientific Research
This work was supported by the Ministry of Education, Culture, Science and Technology, Japan (Grant-in-Aid for Scientific Research 23570226 to Y.Z. and 2222004 to F. Murakami). We thank Ryoichiro Kageyama for Nestin-CreER<SUP>T2</SUP> transgenic mice; Toshio Ohshima for Wnt1-Cre mice; Corrinne Lobe for Z/EG transgenic mice; David Wilkinson for Krox20 in situ plasmid; Michael Wegner for Sox10 in situ plasmid; Yasunori Arimatsu for Nestin antibody; Hiroaki Kobayashi, Kazuhiko Nishida, and Peter Karagiannis for their critical reading of this manuscript; and Xun Hong, who was supported by Osaka University Program for the Support of Networking among Present and Future Researchers, for technical support.