Substantial Receptor-induced Structural Rearrangement of Rotavirus VP8*: Potential Implications for Cross-Species Infection
Xing Yu, Rahul Mishra, Gavan Holloway, Mark von Itzstein, Barbara S Coulson, Helen Blanchard
ChemBioChem | WILEY-V C H VERLAG GMBH | Published : 2015
Rotavirus-cell binding is the essential first step in rotavirus infection. This binding is a major determinant of rotavirus tropism, as host cell invasion is necessary to initiate infection. Initial rotavirus-cell interactions are mediated by carbohydrate-recognizing domain VP8* of the rotavirus capsid spike protein VP4. Here, we report the first observation of significant structural rearrangement of VP8* from human and animal rotavirus strains upon glycan receptor binding. The structural adaptability of rotavirus VP8* delivers important insights into how human and animal rotaviruses utilize the wider range of cellular glycans identified as VP8* binding partners. Furthermore, our studies on ..View full abstract
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Awarded by National Health and Medical Research Council of Australia
Awarded by NHMRC
Financial support from the National Health and Medical Research Council of Australia (ID1085596 to H.B., B.S.C., and M.v.I.) is gratefully acknowledged. B.S.C. is a Senior Research Fellow of the NHMRC (ID628319). The authors acknowledge Vi Dang for her assistance in VP8* plasmid production and the MX1 and MX2 beamline scientists at the Australian Synchrotron for their support during X-ray diffraction data collection.