Journal article
Mycoplasma Infection Alters Cancer Stem Cell Properties in Vitro
C Gedye, T Cardwell, N Dimopoulos, BS Tan, H Jackson, S Svobodová, M Anaka, A Behren, C Maher, O Hofmann, W Hide, O Caballero, ID Davis, J Cebon
Stem Cell Reviews and Reports | Published : 2016
Abstract
Cancer cell lines can be useful to model cancer stem cells. Infection with Mycoplasma species is an insidious problem in mammalian cell culture. While investigating stem-like properties in early passage melanoma cell lines, we noted poorly reproducible results from an aliquot of a cell line that was later found to be infected with Mycoplasma hyorhinis. Deliberate infection of other early passage melanoma cell lines aliquots induced variable and unpredictable effects on expression of putative cancer stem cell markers, clonogenicity, proliferation and global gene expression. Cell lines established in stem cell media (SCM) were equally susceptible. Mycoplasma status is rarely reported in public..
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Awarded by Royal Australasian College of Physicians
Funding Acknowledgements
CG was kindly supported by an NHMRC Biomedical Research PhD Scholarship (280925), a MOGA/COSA/Roche HOTT Fellowship and a Royal Australasian College of Physicians Australia Post Fellowship. MA was supported by a Cancer Council of Victoria PhD Scholarship. JC was a Practitioner Fellow of the National Health and Medical Research Council (NHMRC; 487905). IDD was supported by a Victorian Cancer Agency Clinician Researcher Fellowship and was an NHMRC Practitioner Fellow (487907). We acknowledge the support of a Melanoma Research Alliance (MRA) Team science Award and the Victorian State Government Operational Infrastructure Support Program for partial funding of this project.