Journal article

Colonic structural and ion transport abnormalities in suckling rabbits infected with Escherichia coli K12

EV OLoughlin, L Zhe, A Buret, C Bell, RM RobinsBrowne, EJ Elliott

Journal of Pediatric Gastroenterology and Nutrition | LIPPINCOTT WILLIAMS & WILKINS | Published : 1997


BACKGROUND: Escherichia coli K12 is a laboratory strain considered nonpathogenic. The purpose of this study was to examine the effect of E. coli K12 infection on colonic structure and function. METHODS: Suckling rabbits were infected at 10 days of age with 6 x 10(9) CFU E. coli by intragastric inoculation and were examined 4 to 5 days later. Segments of ileum and proximal and distal colon were removed for light and electron microscopy, and NaCl transport was examined in vitro under short-circuited conditions in Ussing chambers. RESULTS: Infection did not cause weight loss or diarrhea. Colonic mucosa was inflamed with infiltration by polymorphonuclear neutrophils mainly in the lamina propria...

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