Journal article
V-atpase activity in the tgn/ee is required for exocytosis and recycling in arabidopsis 15094
L Yu, S Scholl, A Doering, Z Yi, NG Irani, SD Rubbo, L Neumetzler, P Krishnamoorthy, IV Houtte, E Mylle, V Bischoff, S Vernhettes, J Winne, J Friml, YD Stierhof, K Schumacher, S Persson, E Russinova
Nature Plants | NATURE PUBLISHING GROUP | Published : 2015
Abstract
In plants, vacuolar H + -ATPase (V-ATPase) activity acidifies both the trans-Golgi network/early endosome (TGN/EE) and the vacuole. This dual V-ATPase function has impeded our understanding of how the pH homeostasis within the plant TGN/EE controls exo- and endocytosis. Here, we show that the weak V-ATPase mutant deetiolated3 (det3) displayed a pH increase in the TGN/EE, but not in the vacuole, strongly impairing secretion and recycling of the brassinosteroid receptor and the cellulose synthase complexes to the plasma membrane, in contrast to mutants lacking tonoplast-localized V-ATPase activity only. The brassinosteroid insensitivity and the cellulose deficiency defects in det3 were tightly..
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Awarded by Belgian Federal Science Policy Office
Funding Acknowledgements
We thank Yanhai Yin (Iowa State University, Ames, USA) for providing the anti-BES1 antibody, Niko Geldner (Universite de Lausanne, Switzerland) for sharing pBRI1:BRI1-GFP and pHS:BRI1-YFP lines, Daniel Van Damme (VIB-Ghent University, Belgium) for advice in microscopy, and Martine De Cock (Ghent University, Belgium) for help in preparing the manuscript. This work was supported by the Marie Curie Initial Training Network 'BRAVISSIMO' (grant no. PITN-GA-2008-215118) to E.R., the Odysseus program of the Research Foundation-Flanders to J.F., the Max-Planck Gesellschaft to A.D., Y.Z., L.N. and S.P., the European Union Seventh Framework Programme (FP7 2007-2013) under Grant Agreement 263916 (WallTrac, Marie Curie Initial Training Network) to P.K. and the Deutsche Forschungsgemeinschaft (SFB1101 and TPA02) to K.S. Y.L. is indebted to the Belgian Science Policy Office (BELSPO) for a postdoctoral fellowship.