L-Citrulline Protects Skeletal Muscle Cells from Cachectic Stimuli through an iNOS-Dependent Mechanism
Daniel J Ham, Benjamin G Gleeson, Annabel Chee, Dale M Baum, Marissa K Caldow, Gordon S Lynch, Rene Koopman
PLOS ONE | PUBLIC LIBRARY SCIENCE | Published : 2015
Dietary L-citrulline is thought to modulate muscle protein turnover by increasing L-arginine availability. To date, the direct effects of increased L-citrulline concentrations in muscle have been completely neglected. Therefore, we determined the role of L-citrulline in regulating cell size during catabolic conditions by depriving mature C2C12 myotubes of growth factors (serum free; SF) or growth factors and nutrients (HEPES buffered saline; HBS). Cells were treated with L-citrulline or equimolar concentrations of L-arginine (positive control) or L-alanine (negative control) and changes in cell size and protein turnover were assessed. In myotubes incubated in HBS or SF media, L-citrulline im..View full abstract
Awarded by Ajinomoto Amino Acid Research Program
This work was funded by a grant from the Ajinomoto Amino Acid Research Program (3ARP: 09/07). D.J. Ham & M.K. Caldow were funded by an ESPEN postdoctoral fellowship.