Journal article

Fast, Ultrasensitive Detection of Reactive Oxygen Species Using a Carbon Nanotube Based-Electrocatalytic Intracellular Sensor

Frankie J Rawson, Jacqueline Hicks, Nicholas Dodd, Wondwossen Abate, David J Garrett, Nga Yip, Gyorgy Fejer, Alison J Downard, Kim HR Baronian, Simon K Jackson, Paula M Mendes

ACS APPLIED MATERIALS & INTERFACES | AMER CHEMICAL SOC | Published : 2015

Abstract

Herein, we report a highly sensitive electrocatalytic sensor-cell construct that can electrochemically communicate with the internal environment of immune cells (e.g., macrophages) via the selective monitoring of a particular reactive oxygen species (ROS), hydrogen peroxide. The sensor, which is based on vertically aligned single-walled carbon nanotubes functionalized with an osmium electrocatalyst, enabled the unprecedented detection of a local intracellular "pulse" of ROS on a short second time scale in response to bacterial endotoxin (lipopolysaccharide-LPS) stimulation. Our studies have shown that this initial pulse of ROS is dependent on NADPH oxidase (NOX) and toll like receptor 4 (TLR..

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University of Melbourne Researchers

Grants

Awarded by Leverhulme Trust


Awarded by BBSRC


Awarded by Royal Society


Awarded by Wellcome Trust


Awarded by EPSRC


Awarded by ERC (Consolidator Grant)


Awarded by NC3Rs


Awarded by Biotechnology and Biological Sciences Research Council


Awarded by Engineering and Physical Sciences Research Council


Awarded by National Centre for the Replacement


Funding Acknowledgements

We thank the Leverhulme Trust (F/00 094/BD and ECF/2013-603), BBSRC (BB/L017059/1), Royal Society (RG110087), Wellcome Trust (WT091285MA), EPSRC (EP/K027263/1), ERC (Consolidator Grant 614787), and NC3Rs (NC/L00058X/1) for financial support.