Analysis of the Arabidopsis Cytosolic Proteome Highlights Subcellular Partitioning of Central Plant Metabolism
Jun Ito, Tanveer S Batth, Christopher J Petzold, Alyssa M Redding-Johanson, Aindrila Mukhopadhyay, Robert Verboom, Etienne H Meyer, A Harvey Millar, Joshua L Heazlewood
Journal of Proteome Research | AMER CHEMICAL SOC | Published : 2011
The plant cell cytosol is a dynamic and complex intracellular matrix that, by definition, contains no compartmentalization. Nonetheless, it maintains a wide variety of biochemical networks and often links metabolic pathways across multiple organelles. There have been numerous detailed proteomic studies of organelles in the model plant Arabidopsis thaliana, although no such analysis has been undertaken on the cytosol. The cytosolic protein fraction from cell suspensions of Arabidopsis thaliana was isolated and analyzed using offline strong cation exchange liquid chromatography and LC-MS/MS. This generated a robust set of 1071 cytosolic proteins. Functional annotation of this set revealed majo..View full abstract
Awarded by U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research
This work was part of the DOE Joint BioEnergy Institute (http://www.jbei.org) supported by the U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research, through contract DE-AC02-05CH11231 between Lawrence Berkeley National Laboratory and the U.S. Department of Energy. A.H.M. is supported by the Australian Research Council (ARC) as an Australian Professorial Fellow and by the ARC Centre of Excellence in Plant Energy Biology. We are grateful to Prof. Stephen Fry and the Edinburgh Cell Wall Group (University of Edinburgh) for providing the Arabidopsis cell culture. We also thank Anongpat Suttangkakul (Joint BioEnergy Institute) for her assistance with ubiquitin/26S proteasome nomenclature.