The Arabidopsis thaliana 2-D gel mitochondrial proteome: Refining the value of reference maps for assessing protein abundance, contaminants and post-translational modifications
Nicolas L Taylor, Joshua L Heazlewood, A Harvey Millar
Proteomics | WILEY | Published : 2011
Mitochondria undertake the process of oxidative phosphorylation yielding ATP for plant cell maintenance and growth. The principles of isolation and fractionation of plant mitochondrial proteins have been improved over decades, and surveys of the mitochondrial proteome in a number of plants species have been performed. Over time, many quantitative analyses of changes in the plant mitochondrial proteome have been performed by 2-D gel analyses revealing the induction, degradation and modification of mitochondrial proteins in responses to mutation, stress and development. Here, we present a saturating MS analysis of 2-D gel separable protein spots from a typical purification of Arabidopsis mitoc..View full abstract
Awarded by United States Department of Energy, Office of Science, Office of Biological and Environmental Research, through the Joint BioEnergy Institute
This work was supported by the Australian Research Council (ARC) through an Australian Professorial Fellow (AHM) and by the ARC Centre of Excellence in Plant Energy Biology. This work was also supported by the United States Department of Energy, Office of Science, Office of Biological and Environmental Research, through the Joint BioEnergy Institute (contract DE-AC02-05CH11231) between Lawrence Berkeley National Laboratory and the U. S. Department of Energy.