Journal article

Analysis of the Arabidopsis cytosolic ribosome proteome provides detailed insights into its components and their post-translational modification

Adam J Carroll, Joshua L Heazlewood, Jun Ito, A Harvey Millar

MOLECULAR & CELLULAR PROTEOMICS | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | Published : 2008

Abstract

Finding gene-specific peptides by mass spectrometry analysis to pinpoint gene loci responsible for particular protein products is a major challenge in proteomics especially in highly conserved gene families in higher eukaryotes. We used a combination of in silico approaches coupled to mass spectrometry analysis to advance the proteomics insight into Arabidopsis cytosolic ribosomal composition and its post-translational modifications. In silico digestion of all 409 ribosomal protein sequences in Arabidopsis defined the proportion of theoretical gene-specific peptides for each gene family and highlighted the need for low m/z cutoffs of MS ion selection for MS/MS to characterize low molecular w..

View full abstract

University of Melbourne Researchers