Journal article

Proteomic identification of divalent metal cation binding proteins in plant mitochondria

VL Herald, JL Heazlewood, DA Day, AH Millar

FEBS LETTERS | ELSEVIER SCIENCE BV | Published : 2003

Abstract

Divalent metal binding proteins in the Arabidopsis mitochondrial proteome were analysed by mobility shifts in the presence of divalent cations during two-dimensional diagonal sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Tandem mass spectrometry and searches of the predicted Arabidopsis protein dataset were used in an attempt to identify 34 of the proteins which shifted. This analysis identified a total of 23 distinct protein spots as the products of at least 11 different Arabidopsis genes. A series of proteins known to be divalent cation-binding proteins, or to catalyse divalent cation-dependent reactions, were identified. These included: succinyl CoA ligase beta subunit, Mn-s..

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University of Melbourne Researchers