The Amniotic Fluid Transcriptome A Source of Novel Information About Human Fetal Development
Lisa Hui, Donna K Slonim, Heather C Wick, Kirby L Johnson, Diana W Bianchi
Obstetrics and Gynecology | LIPPINCOTT WILLIAMS & WILKINS | Published : 2012
OBJECTIVE: Amniotic fluid is a complex biological material that provides a unique window into the developing human. Residual amniotic fluid supernatant contains cell-free fetal RNA. The objective of this study was to develop an understanding of the amniotic fluid core transcriptome by analyzing the transcripts ubiquitously present in the amniotic fluid supernatant of euploid midtrimester fetuses. METHODS: This was an in silico (computational) investigation using publicly available gene expression data previously produced by our group from 12 euploid midtrimester amniotic fluid samples. Functional analyses were performed using a web-based software analysis tool. Organ specificity was examined..View full abstract
Awarded by Eunice Kennedy Shriver National Institute of Child Health and Human Development
Awarded by EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT
Supported by the Eunice Kennedy Shriver National Institute of Child Health and Human Development (R01 HD 42053-08 to Dr Bianchi and R01 HD 058880 to Dr Slonim); the University of Sydney Medical School (Albert S. McKern Research Scholarship to Dr Hui); and the Royal Australian and New Zealand College of Obstetricians and Gynaecologists Research Foundation (Fotheringham Fellowship to Dr Hui).