Journal article

Deconvoluting the Effect of the Hydrophobic and Hydrophilic Domains of an Amphiphilic Integral Membrane Protein in Lipid Bicontinuous Cubic Mesophases

Leonie van't Hag, Hsin-Hui Shen, Jingxiong Lu, Adrian M Hawley, Sally L Gras, Calum J Drummond, Charlotte E Conn



Lipidic bicontinuous cubic mesophases with encapsulated amphiphilic proteins are widely used in a range of biological and biomedical applications, including in meso crystallization, as drug delivery vehicles for therapeutic proteins, and as biosensors and biofuel cells. However, the effect of amphiphilic protein encapsulation on the cubic phase nanostructure is not well-understood. In this study, we illustrate the effect of incorporating the bacterial amphiphilic membrane protein Ag43, and its individual hydrophobic β(43) and hydrophilic α(43) domains, in bicontinuous cubic mesophases. For the monoolein, monoalmitolein, and phytantriol cubic phases with and without 8% w/w cholesterol, the ef..

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University of Melbourne Researchers


Awarded by ARC Dairy Innovation Hub

Funding Acknowledgements

We thank Dr. Stephen T. Mudie and Dr. Nigel M. Kirby for their assistance with SAXS experiments, and we acknowledge use of the SAXS/WAXS beamline at the Australian Synchrotron. We thank Dr. Janet Newman and Dr. Shane A. Seabrook for help with the robotic setup of plates at the C<SUP>3</SUP> Collaborative Crystallization Centre, CSIRO, Parkville, Australia. We acknowledge Dr. Yee-Foong Mok for help with CD measurements at the Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, Australia. Sally L. Gras is supported by the ARC Dairy Innovation Hub IH120100005.