Prolonged use of tenofovir in HIV/hepatitis B virus (HBV)-coinfected individuals does not lead to HBV polymerase mutations and is associated with persistence of lamivudine HBV polymerase mutations
J Audsley, N Arrifin, LKW Yuen, A Ayres, SM Crowe, A Bartholomeusz, SA Locarnini, A Mijch, SR Lewin, J Sasadeusz
HIV Medicine | WILEY | Published : 2009
OBJECTIVES: The aim of the study was to identify and characterize hepatitis B virus (HBV) polymerase gene mutations associated with ongoing HBV replication in HIV/HBV-coinfected individuals receiving tenofovir (TDF). METHODS: This retrospective cross-sectional study identified 28 HIV/HBV-coinfected individuals who had received TDF for at least 3 months. All patients had samples available while receiving TDF (on-TDF), and 24 also had samples available prior to treatment (pre-TDF). Case records were reviewed to obtain clinical and virological data at the times of sampling (+/-3 months). The HBV DNA of all samples was amplified using polymerase chain reaction (PCR), and the polymerase region of..View full abstract
Awarded by National Institute of Health
The authors thank the participants in the study, Professor Jenny Hoy, the Clinical Research nursing staff, Kerry Watson and Scott Bowden for assistance with participant recruitment and sample analysis. We acknowledge funding from the NHMRC Centre for Clinical Research Excellence in Infectious Diseases, The University of Melbourne and the National Institute of Health (grant RO1 A1060449).