Fractionation of whey proteins from red deer (Cervus elaphus) milk and comparison with whey proteins from cow, sheep and goat milks
Minh Ha, Alaa El-Din Bekhit, Michelle McConnell, Sue Mason, Alan Carne
Small Ruminant Research | ELSEVIER SCIENCE BV | Published : 2014
In the present study, we report the first protein fractionation analysis of whey proteins from red deer (Cervus elaphus) milk. The aim of this study was to investigate the protein composition of red deer milk and examine the protein profile compared with that of cow, sheep and goat using 1D-PAGE and protein RP-HPLC of defatted milk. Ammonium sulfate fractionated sweet whey proteins of the four species were further subjected to anion exchange chromatography, and 1D- and large format 2D-PAGE analysis. Deer milk caseins displayed different mobility on 1D-PAGE, and chromatographed differently on RP-HPLC to those of other species. The apparent ratio of alpha-lactalbumin to beta-lactoglobulin in r..View full abstract
We thank the Lincoln University deer farm, Lincoln, New Zealand, for supplying red deer whole milk, and local farm supply of cow, sheep and goat milk. M. Ha acknowledges receipt of a University of Otago PhD Scholarship.