Journal article

Cytokine dialysis: An ex vivo study

S Uchino, R Bellomo, H Morimatsu, D Goldsmith, P Davenport, L Cole, I Baldwin, S Panagiotopoulos, P Tipping, S Morgera, HH Neumayer, H Goehl

ASAIO Journal | LIPPINCOTT WILLIAMS & WILKINS | Published : 2002

Abstract

To test the hypothesis that dialysis using a new large pore membrane would achieve effective cytokine removal, blood from six volunteers was incubated with endotoxin (1 mg) and then circulated through a closed circuit with a polyamide membrane (nominal cut-off: 100 kDa). Hemodialysis was conducted at 1 or 9 L/hr of dialysate flow at the start of circulation and after 2 and 4 hours. The peak dialysate/plasma concentration ratios were 0.92 for interleukin (IL)-1beta, 0.67 for IL-6, 0.94 for IL-8, 0.33 for tumor necrosis factor (TNF)-a, and 0.11 for albumin. The dialysate/plasma ratios for all cytokines and albumin were decreased with increased dialysate flow from 1 to 9 L/hr (p < 0.05). Cleara..

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