Detection of Hepatitis B Virus DNA in Tears by Polymerase Chain Reaction

Abstract

Objective: To assess the possibility of transmission of hepatitis B virus (HBV) via tears. Methods: Tear and plasma specimens were collected from 36 carriers of HBV, and the presence of HBV DNA was investigated using the polymerase chain reaction (PCR). Results: Ten (29.4%) of the 34 carriers of chronic HBV and one of the two patients with acute hepatitis B had plasma specimens that were positive for HBV DNA using PCR. Sixteen (47.1%) of the 34 carriers of chronic HBV had tear specimens that were repeatedly positive for HBV DNA using PCR, 10 (29.4%) had tear specimens that were repeatedly negative, and the remaining eight (23.5%) had tear specimens that were equivocal. Both of the patients w..