Journal article

H-1-NMR analysis of water mobility in cultured phototrophic biofilms

F Di Pippo, A Bohn, F Cavalieri, P Albertano



The present work reports on the first attempt to study water mobility in phototrophic biofilms, applying the (1)H-NMR relaxometry technique to closely monitored microbial communities grown in a microcosm under controlled ambient conditions. Longitudinal water proton relaxation times exhibited a bi-exponential behavior in all biofilm samples, indicating two types of water molecules with diverging dynamic properties, confined to different compartments of the biofilm. The fast-relaxing component can be attributed to water molecules tightly bound to the intracellular matrix, while the slow-relaxing component could reflect the behavior of water embedded in the biopolymer matrix, confined into mat..

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University of Melbourne Researchers


Awarded by Fundacao para Ciencia e Technologia, Portugal

Funding Acknowledgements

The authors acknowledge Dr NTW Ellwood for revision of the manuscript and Dr E. Iorio for providing valuable comments and suggestions. This research was partly conducted within the framework of EU project PHOBIA (QLK3-CT2002-01938). AB acknowledges support by the Fundacao para Ciencia e Technologia, Portugal, through project PTDC/BIA-MIC/72512/2006.