Influence of polyelectrolyte multilayer coatings on Förster resonance energy transfer between 6-carboxyfluorescein and rhodamine B-labeled particles in aqueous solution

Abstract

The Förster resonance energy transfer (FRET) between 6-carboxyfluorescein (6-CF) (donor) and rhodamine B-labeled melamine formaldehyde (RhB-MF) particles (acceptor) in aqueous solution was exploited to investigate the layer properties of polyelectrolyte (PE) multilayers preadsorbed on the particle surface. The formation of poly(styrenesulfonate) (PSS) and poly(allylamine hydrochloride) (PAH) multilayers on the RhB-MF particles was confirmed by electrophoretic mobility measurements. The FRET process was found to proceed via adsorption of 6-CF onto the RhB-MF particles and was thus dependent on the degree of surface coverage of the PE on the surface. The PE surface coverage could be altered by..