Journal article
Enhanced enzymatic degradation resistance of plasmid DNA in ionic liquids
RR Mazid, A Cooper, Y Zhang, R Vijayaraghavan, DR MacFarlane, C Cortez-Jugo, W Cheng
Rsc Advances | ROYAL SOC CHEMISTRY | Published : 2015
DOI: 10.1039/c5ra05518k
Abstract
Plasmid deoxyribonucleic acid (pDNA) is a promising therapeutic in gene therapy and as a DNA vaccine, but it is susceptible to degradation by nucleases in the extracellular environment. In this study, we report on the enhanced enzymatic degradation resistance of pDNA, when stored in a hydrated buffered ionic liquid (BIL) based on choline dihydrogen phosphate (CDHP). The stability of pDNA stored in buffered CDHP (bCDHP) in the presence of Turbo DNase was studied using agarose gel electrophoresis, which showed a prolonged shelf life at room temperature over a period of 28 days in bCDHP compared to within 10 minutes for phosphate buffered saline (PBS). In addition, the biological activity of pD..
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Funding Acknowledgements
Australia Discovery Projects [120100170]. CCJ is funded through an Australian Research Council (ARC) Super Science Fellowship through grant FS100100073. CCJ acknowledges the Melbourne Centre for Nanofabrication for the Technology Fellowship. DRM is grateful to the ARC for his Laureate Fellowship funding. R.R.M acknowledges the Monash ChemEng Departmental scholarship. This work was performed in part at the Melbourne Centre for Nanofabrication (MCN) in the Victorian Node of the Australian National Fabrication Facility (ANFF).