Deep sequencing of the uterine immune response to bacteria during the equine oestrous cycle
CD Marth, ND Young, LY Glenton, DM Noden, GF Browning, N Krekeler
BMC Genomics | BioMed Central | Published : 2015
© 2015 Marth et al. Background: The steroid hormone environment in healthy horses seems to have a significant impact on the efficiency of their uterine immune response. The objective of this study was to characterize the changes in gene expression in the equine endometrium in response to the introduction of bacterial pathogens and the influence of steroid hormone concentrations on this expression. Methods: Endometrial biopsies were collected from five horses before and 3 h after the inoculation of Escherichia coli once in oestrus (follicle > 35 mm in diameter) and once in dioestrus (5 days after ovulation) and analysed using high-throughput RNA sequencing techniques (RNA-Seq). Results: Compa..View full abstract
This work was supported by funds from an Early Career Research Grant provided by the University of Melbourne and the Department of Biomedical Sciences, Cornell University College of Veterinary Medicine, Ithaca, New York.